摘要
该文以胡杨 (Populuseuphratica)悬浮细胞为材料 ,利用 1 0 %纤维素酶“onozuka”R 10、0 0 1%果胶酶Y 2 3、0 15 %离析酶R 10和 0 1%半纤维素酶的混合酶液消化细胞壁 ,得到原生质体 .并利用膜片钳细胞贴附技术分别测到质膜内向和外向单通道电流 .通道电流在不同水平上变化 ,表明并非只有一种类型的通道开放 .
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.
基金
SupportedinpartbytheNationalNaturalScienceFoundationofChina(NNSF) (GrantNo .30 0 70 6 13)
theFoundationfortheAuthorsofNationalExcellentDoctoralDissertationofP .R .China(GrantNo .2 0 0 15 2 )
Nation alLaboratoryofBiomembranesandMembraneBiotech
关键词
胡杨
悬浮细胞
原生质体
膜片钳
细胞贴附技术
单通道
Populus euphratica, suspension cells, protoplasts, patch\|clamp, cell\|attached recording technique, single ion channel
