摘要
目的了解福建省宁化林区斑点热自然疫源地存在情况。方法用聚合酶链反应(PCR)对该林区鼠、蜱类中感染斑点热群立克次体(sFGR)进行扩增。用限制性酶切片段长度多态分析(RFLP)法对分离株 NH-97进行鉴定,并同已知的西伯利亚立克次体等国际标准株进行比较。结果从越原血蜱、金泽革蜱、微小牛蜱中扩增出 SFGR 特异的 DNA 片段,NH-97分离株的 PCR 产物经 PstI 和 RsaI 酶切后发现它们的酶切图谱与西伯利亚立克次体246国际标准株相同。从社鼠,黄胸鼠脏器中扩增出康氏立克次体 DNA 片段。由不同来源批次的越原血蜱中扩增出近缘于日本立克体 DNA 片段和相关序列提示:可能是斑点热群立克次体新成员。结论福建宁化林区除存在西伯利亚立克次体外,还可能存在康氏立克次体,日本立克次体等多种斑点热群立克次体的疫源地。
Objective To study on existing nature foci of Spotted Fever in Ninghua,Fujian.Methods Using DNA polymerase chain reaction and restriction endonuclease fragment length polymorphism analysis(PCR/ RFLP) to detect spotted fever group rickettsiae(SFGR) in ticks.Results It showed that H.wellingtoni,H.yeni, Dermacentor auratus were infected with Rickettsia sibirica,the DNA productions were cloned,NH-97 was anti- genically and genotypically identical to Riskettsia sibirica.Rattus flavipectus were found infected R.conorii.One of the sequeuce analysis showed that the DNA sequence was different form other SFGR and close to R.japanic.Conclusion It exists nature foci of R.sibirica,R.sibrica,R.japanic and R.conorii in Ninghua,Fujian province of China.
