检测胞浆抗原用于急性白血病免疫分型

IMMUNOPHENOTYPING OF ACUTE LEUKEMIA BY MEASURING CELL PLASMA ANTIGENS

目的:探讨胞浆抗原在急性白血病免疫分型中的临床意义。方法:用流式细胞仪检测42例初治急性白血病患者胞浆抗原CyCD_3,CyCD_(22),CyCD_(79a)和MPO。结果:T—ALL阳性胞浆抗原主要表达在CyCD_3,阳性率占88.9％。B—ALL阳性胞浆抗原主要表达在CyCD_(79a),AML主要表达CyMPO。结论:CyCD_3和CyCD_(79a),及MPO可分别作为T—ALL,B—ALL和AML系列特异性标志,对正确诊断白血病提供非常有价值的依据。

Objective:To evaluate the role of the cell plasma antigens in acute leukemia immunophenotyping. Methods: The cell plasma antigens that included CyCD3, CyCD22, CyCD79a and MPO were detected in 42 patients with acute leukemia by flow cytometer. Results:The CyCD3 expression was observed mainly in T- ALL cell plasma antigen with 88.9% postive rate. The CyCD79a and CyMPO expressed mainly in B - ALL and AML, respectively. Conclusion :CyCD3,CyCD79a and MPO can act as sequences of specific mark for T - ALL,B- ALL and AML respectively, which will benefit the diagnosis of acute leukemia.