中加燕麦种质的遗传多样性和群体结构分析

Genetic Diversity and Population Structure Analysis of Oat Germplasm from China and Canada

为揭示燕麦种质资源的遗传基础,采用内含子切接点引物和长随机引物的PCR分子标记技术,对加拿大和中国的64份燕麦种质的遗传多样性和群体结构进行了分析。结果表明,所用15条引物共扩增出171条带,平均每条引物扩增出11.4条带,其中多态性带共134条,多态性百分比为78.36%。燕麦各种质间的Dice遗传相似系数分析表明,所有燕麦材料间的遗传相似系数变幅为0.699~0.934,平均值为0.81,表明参试燕麦种质间存在着一定的遗传差异,但遗传差异相对较小。群体结构分析结果表明,所研究的燕麦种质中81.25%的种质血缘相对比较单一,仅18.75%的种质拥有混合来源。

To understand the genetic basis of oat(Avena sativa L.) germplasm,the genetic diversity and population structure among 64 oat accessions from China and Canada were analyzed using PCR with intron-splice junction primers and long random primers.171 distinct PCR bands were amplified in total of 15 primers,and 11.4 bands per primer was amplified.Among the 171 bands amplified,134 were polymorphic,and the polymorphic rate was 78.36%.The Dice genetic similarity coefficients among those germplasm ranged in 0.699～0....