摘要
建立了同时检测葡萄酒中8种有机酸的高效液相色谱分析方法。采用日本资生堂CAPCELLPAKMGS5色谱柱(4.6mmi.d.×150mm,5μm),乙睛-磷酸溶液为流动相等度洗脱,流速为0.5mL/min,210nm和243nm下检测,外标定量法。结果显示:8种有机酸的线性关系良好,相关系数在0.9953~0.9999之间;精密度、稳定性的RSD都低于5%;平均回收率为85%~120%之间,检出限为0.0141~1.3427mg/L之间。
A method was established to determine eight organic acids in wine by reversed phase high performance liquid chromatography (RP- HPLC). The HPLC optimal conditions contained: Japan CAPCELL PAK MG S5,(4.6mmi.d. ×150 mm, 5μm), a mobile phase gradient elution with acetonitrile- phosphoric acid, the flow rate of 0.5 ml/min, the detect wave at 210 nm and 243 nm. The quantified analysis of external standard calibration curves was used. There were good linear correlations and the correlation coefficient were 0.9953...
出处
《酿酒》
CAS
2010年第4期62-65,共4页
Liquor Making
基金
教育部高等学校特色专业建设项目
项目号TS10618
