摘要
建立了柱前衍生高效液相色谱法同时测定黄酒中17种氨基酸的方法。方法采用异硫氰酸苯酯(PITC)为柱前衍生试剂,色谱柱为C18(4.6×250mm,5μm),乙酸钠-乙腈-水混合体系梯度洗脱,紫外检测器,检测波长254nm,40min内17种氨基酸得到良好的分离。在0.025～1.25mmol/L范围内其浓度与响应值具有良好的线性关系,相关系数大于0.99;样品的平均加标回收率在78.6～118.1%之间,相对标准偏差在0.45%～3.30%范围;检出限为0.259～0.925μmol/L,定量限为0.863～3.083μmol/L。相对其他柱前衍生测定氨基酸的方法而言,该方法在可操作、低成本、易推广等方面具有明显优势,可供黄酒中氨基酸测定方法行业标准制定提供参考。
A reversed-phase high performance liquid chromatographic(HPLC) method for simultaneous determination of 17 amino acids in Chinese rice wine with pre-column derivation was developed.The sample was derived with phenylisothiocyanate(PITC) and analyzed on C18 column(4.6×250mm,5μm) with detection at 254nm..Using this method,17 amino acids can be separated well in 40min by gradient elution with mix mobile phase of acetonitrile,sodium acetate solution and water.The linear range investigated in this method was 0.02...
出处
《酿酒》
CAS
2010年第5期74-76,共3页
Liquor Making
关键词
黄酒
柱前衍生
异硫氰酸苯酯
氨基酸
标准
Chinese rice wine
pre-column derivation
phenylisothiocyanate（PITC）
amino acids
standard
