正常人肠道肠球菌esp基因与生物膜形成关系的初探

The Relationship Between esp and the Ability of Biofilm Formation in Enterococci from the Intestinal Tract of Normal People

目的了解正常人肠道肠球菌生物膜形成情况,探讨肠球菌esp基因与生物膜形成的关系。方法用PCR法检测肠球菌esp基因,用96孔聚苯乙烯板进行生物膜形成试验。结果 84株正常人肠道肠球菌esp基因的检出率为14.3%,生物膜形成阳性率为25.0%,其中esp基因阳性株的生物膜形成率为83.3%,esp基因阴性株的生物膜形成率仅为15.3%。结论正常人肠道肠球菌形成生物膜的能力较弱,esp基因是肠球菌形成生物膜的重要因素,生物膜的形成不局限于esp阳性菌株。

Objective To investigate the biofilm formation of Enterococci from the intestinal tract of normal people,to study the relationship between esp and the ability of biofilm formation in Enterococci.Methods PCR was applied to detect the esp gene in 84 isolates.And the biofilm formation was performed in 96 well polystyrene plates. Results The detection rate of esp in Enterococci was 14.3%,the rate of biofilm formation was 25.0%,the rate of biofilm formation of esp positive isolates was 83.3%,and that of esp negative isolates was 15.3%.Conclusion Biofilm formation is not very common in Enterococci from the intestinal tract of normal people,esp is an important factor of biofilm formation,and the ability of biofilm formation is not limited to the esp positive isolates.