内脂素对大鼠肝星状细胞表达碱性成纤维细胞生长因子的影响

Effect of Visfatin on the Expression of Basic Fibroblast Growth Factor in Rat Hepatic Stellate Cells

目的观察内脂素对大鼠肝星状细胞(HSCs)表达碱性成纤维细胞生长因子(FGF-2)的影响,探讨内脂素在肝纤维化中的作用。方法对培养激活的大鼠HSCs分别给予不同浓度(0、50、100和200 ng/mL)的内脂素进行刺激,在不同时限(6 h、12 h、24 h及36 h)收集细胞总RNA。用实时荧光定量(SYBR GreenⅠ)RT-PCR检测FGF-2 mRNA的表达。结果 50 ng/mL内脂素孵育HSCs 24 h可上调FGF-2 mRNA的表达(1.71±0.34 vs.1,P<0.05),随着刺激浓度的增加FGF-2 mRNA的表达进一步上调(P<0.05)。100 ng/mL内脂素孵育HSCs 12h可促进FGF-2 mRNA的表达(1.68±0.31 vs.1,P<0.05),随着刺激时间的延长,FGF-2 mRNA的表达逐渐增加(P<0.05)。结论内脂素能诱导大鼠HSCs表达FGF-2,提示内脂素可能通过诱导FGF-2的表达参与肝纤维化的发病过程。

Objective To investigate the effect of visfatin on the expression of basic fibroblast growth factor(FGF-2) in rat hepatic stellate cells(HSCs).Methods Culture-activated rat HSCs were incubated with visfatin under different dose(50,100,200 ng/mL) for 6,12,24 and 36 hours respectively.Total RNA were collected at each time point.FGF-2 mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction.Results The mRNA expression of FGF-2 was dose-dependently up-regulated in rat HSCs when incubated with 50,100 and 200 ng/mL visfatin(1.71±0.34 vs.1,2.25±0.26 vs.1,3.25±0.29 vs.1,P< 0.05,respectively).FGF-2 mRNA expression also increased after 100 ng/mL visfatin treatment for 12 hours(1.68±0.31 vs.1,P< 0.05),and the mRNA levels were time-dependently elevated from 24 hours to 36 hours(2.25±0.26 vs.1,2.95±0.32 vs.1,P<0.05,respectively).Conclusion Visfatin could induce FGF-2 mRNA expression in rat HSCs in a dose-and time-dependent manner.Visfatin might be involved in the pathogenesis of liver fibrosis.