GluR5、GluR6在马桑内酯与ATPA致痫的大鼠星形胶质细胞中的不同表达

Different Expression of GluR5 and GluR6 in Rat Astrocyte Induced with Coriaria Lactone and ATPA

目的探讨GluR5、GluR6在马桑内酯与ATPA致痫的大鼠星形胶质细胞中的不同表达及其在致痫模型中可能的作用机制。方法采用ATPA和马桑内酯(coriaria lactone,CL)分别诱导处理大鼠星形胶质细胞,加入等量生理盐水培养的星形胶质细胞作为对照组,通过RT-PCR和Western blot检测细胞中GluR5、GluR6的mRNA和蛋白质水平。结果 ATPA作用后星形胶质细胞GluR5的mRNA和蛋白质表达较对照组升高(P<0.05),GluR6的表达较对照组无明显变化(P>0.05);CL作用后星形胶质细胞GluR5和GluR6表达均明显降低(P<0.05)。结论 ATPA上调星形胶质细胞的GluR5受体,而CL下调星形胶质细胞的GluR5、GluR6受体,提示GluR5、GluR6在不同致痫剂诱导的癫痫活动中有不同的作用机制,CL对GluR5和GluR6可能存在负反馈调节,具体分子机制尚需进一步研究。

Objective To investigate the different expression of GluR5 and GluR6 in rat astrocyte induced with Coriaria Lactone(CL) and ATPA and to discuss the possible mechanism of the inducing epileptic model.Methods Astrocyte of neonate rats was treated with CL and ATPA respectively and the astrocyte treated with normal saline as control.The mRNA and protein levels of GluR5 and GluR6 were detected by RT-PCR and western blotting.Results Compared with the control,the expression of GluR5 was increased significantly in the astrocyte induced by ATPA(P<0.05),however,there was no alteration of GluR6 expression.The expression of GluR5 and GluR6 were decreased significantly in CL-treated group(P<0.05).Conclsuion The present study showed ATPA up-regulated mRNA and protein levels of GluR5,but coriaria lactone down-regulated the GluR5 and GluR6 of astrocyte.The results indicate that there are different pathologic mechanisms of GluR5 and GluR6 in different neurotoxicants inducing model of epilepsy.The molecular mechanism need to be studied further in the future.