痛风性关节炎大鼠模型尿液的代谢组学研究

Metabonomic Study of the Urine of Rat Acute Gouty Arthritis Model

目的通过代谢组学研究,了解痛风性关节炎大鼠模型尿液中代谢组的变化规律。方法基于代谢组学方法,以LC/MS为数据采集平台,并结合主成分分析方法等化学计量学手段,研究空白大鼠、痛风性关节炎大鼠尿液中代谢物组时量的轨迹变化。结果造模后大鼠的代谢状态相对其造模前及空白对照组大鼠出现了偏离,随着时间的推移又逐渐回复到造模前的初始代谢状态,以此代谢组学方法所得的大鼠代谢轨迹变化很好地反映了大鼠痛风性关节炎的发生、发展、恢复的病程状况。结论模型的整体代谢状态通过代谢组学方法可以得到很好的体现,因而代谢组学技术为痛风性关节炎模型提供了良好的研究手段。

Objective To analysis the metabonome characteristics of the urine of MSU crystal-induced acute gouty arthritis rats by the method of metabolomics.Methods Based on the method of metabolomics, which applies LC/MS as data acquisition platform,incorporating with the means of stoechiometry such as principal component analysis,we analyzed the metabonome difference between the urine of acute gouty arthritis rats and that of normal rats.Results Compare with control group,the metabolism status of acute gouty arthritis model group deviated.After that,with the time lapsed,it retrieved gradually to the incipient metabolism status.The variation of metabolism locus of rats measured by the methods of metabolomics properly reflects the genesis,development,and recuperation course of acute gouty arthritis.Conclusion The whole metabolism status of rat model is able to be presented finely with the method of metabolomics.The metabolomics study could offer a satisfactory research approach to acute gouty arthritis.