头颈部鳞癌人乳头状瘤病毒16/18感染状态与Ki-67、P53表达的关系

Infection of Human Papillomavirus 16/18 DNA in Patients with Head and Neck Squamous Cell Carcinoma and Its Relationship with Expression of Ki-67 and P53 Protein

目的通过测定人头颈部鳞状细胞癌组织中人乳头状瘤病毒(HPV)16/18感染和Ki-67、P53蛋白的表达,探讨HPV16/18感染和Ki-67、P53蛋白表达的相关性及意义。方法采用荧光定量PCR方法测定头颈部鳞癌患者肿瘤组织中HPV16/18DNA的含量,同时以免疫组织化学方法测定Ki-67、P53蛋白的表达状态。结果 62.8%的患者在头颈部鳞癌组织中检测到HPV16/18DNA,46.15%的患者肿瘤组织表达P53蛋白为阳性。患者肿瘤组织Ki-67的表达在2%～70%。HPV16/18DNA含量与患者的性别、吸烟、饮酒和临床分期无相关性(P>0.05),与病理学分级呈正相关(r=0.350,P=0.001),与Ki-67表达无相关性(P=0.179),与P53表达呈负相关(r=-0.197,P=0.04)。结论头颈部鳞癌的发生可能和HPV16/18感染有关,致病机制涉及P53蛋白的降低表达。

Objective To detect the infection of human papillomavirus(HPV)16 /18 in patients with head and neck squamous cell carcinoma and explore the relationship between HPV infection and expressions of Ki-67 and P53 proteins in tumor tissue.Method The level of HPV 16 /18 DNA was measured by real time polymerase chain reaction,and Ki-67 and P53 proteins were measured by immunohistochemistry in tissues from head and neck squamous cell carcinoma.Results HPV 16 /18 DNA was detected in 62.8% of our patients.In each cancer tissue sample,Ki-67 protein was expressed between 2% to 70%.P53 protein was expressed in 46.15% of our patients.No significant relation was found between HPV 16 /18 DNA level and sex,smoking,drinking,and tumor clinical stages.However,level of HPV 16 /18 DNA was found to have positive relation with tumor pathological grades and negative relation with P53 protein expression.No relation with Ki-67 protein expression was found.Conclusion Head and neck squamous cell carcinoma may be initiated by HPV 16 /18 infection and the mechanism in carcinogenesis involves abnormal expression in P53 protein.