重组人p53腺病毒联合顺铂对肺腺癌A549细胞基因表达的影响

Effect of Recombinant Human Adenovirus p53 Combined with Cisplatin on the Expression of Human Lung Adenocarcinoma A549 Cell Gene

目的研究重组人p53腺病毒(rAd-p53)联合顺铂对肺腺癌A549细胞基因表达的影响,探讨rAd-p53增强顺铂对A549细胞抑制作用的机制。方法通过基因芯片检测技术,比较rAd-p53联合顺铂与单独顺铂处理后的肺腺癌A549细胞肿瘤相关基因表达的差异,采用SAM软件对结果进行分析。结果 rAd-p53联合顺铂与单独顺铂处理的A549细胞比较,15个基因表达显著上调,28个基因表达显著下调。结论 rAd-p53增强顺铂对A549细胞的抑制作用与导入外源性p53基因后,p53基因表达上调并引起调控细胞周期、细胞增殖、细胞凋亡的一系列基因的表达变化。

Objective To explore the effect of recombinant human adenovirus p53(rAd-p53)com-bined with cisplatin on the expression of human lung adenocarcinoma A549 cells.Methods Human lung ade-nocarcinoma A549 cells were treated with rAd-p53 combined with cisplatin(combination group)or with cispl-atin alone(cisplatin group).The expressions of the cell genes were compared between these two groups and the results were analyzed by SAM software.Result A total of 43 differential genes were found,15 of which were up-regulated and 28 were down-regulated.Conclusion Following introduction of rAd-p53,many genes regulating cell cycle,proliferation and apoptosis expresses up or down which significantly enhance chemosensi-tivity and killing efficiency of cisplatin on human lung adenocarcinoma A549 cells.