壳聚糖与聚乙二醇交联水凝胶研究

Study on a Chitosan and Polyethylene Glycol Crosslinked Hydrogel

研究对壳聚糖(CS)进行化学修饰得到了不同丙烯酰基取代度(1.03%,3.55%和5.21%)的丙烯酰化羟丙基壳聚糖(AHCS);通过自由基引发反应,AHCS与聚乙二醇二丙烯酸酯(PEGDA)交联得到壳聚糖(CS)与聚乙二醇(PEG)为主体的交联水凝胶。通过SEM观察其为通透性良好的多孔性支架材料。水凝胶的溶胶含量和溶胀度随丙烯酰基取代度的增加而降低,水凝胶中壳聚糖的降解速率也随丙烯酰基取代度的升高而降低。对于同一取代度的交联水凝胶,其在酸性和碱性条件下的溶胀度大于中性环境。细胞试验表明,壳聚糖与聚乙二醇交联水凝胶具有良好的生物相容性。

In this study,acrylated hydroxypropyl chitosan(AHCS) with different substitution degree(SD) of acryloyl,i.e.,1.03%,3.55% and 5.21%,was prepared by chemical modification of chitosan(CS).Through free radical initiation reaction,AHCS and polyethylene glycol diacrylate(PEGDA) were crosslinked to form CS-PEG hydrogel.SEM indicated it was a porous scaffold with high connection rate.Our results suggested that the sol content(SC) and swelling ratio(SR) of the hydrogels decreased with increase of SD,and SR was highe...