微流体芯片动态调控蛋白水溶液pH或Ca^(2+)浓度

Component Adjustments of Silk Fibroin Aqueous Solution Flowing in a Biomimetic Microfluidic Chip

模拟蚕和蜘蛛的纺丝系统,以载玻片(75×25mm)为基片,SU-8为阳模材料,聚二甲基硅氧烷(PDMS)为微通道(宽500μm,深100μm)构筑材料,利用光刻及模塑成型等技术设计、制备了微流体芯片,并应用于再生丝素蛋白(RSF)水溶液组成的动态调控。该芯片利用微流体的层流特性使RSF水溶液与pH缓冲液或钙离子缓冲液在微通道内平行流动而不发生混合。在水溶液中RSF质量分数不变的前提下,通过离子扩散实现了流动场下RSF水溶液pH值或钙离子浓度的动态调控。

In this article,a microfluidic channel(500 μm in width,100 μm in depth) in a micro-chip was fabricated in poly-(dimethylsiloxane)(PDMS) on a SU-8 mold based on photolithography and molding process.By biomimicking the glands and the spinning ducts of silk worm and spider,this chip was designed and used for pH or Ca^(2+) concentration adjustments of regenerated silk fibroin(RSF) aqueous solution.Due to the laminar flow characteristic of microfluidic fluids,the RSF solution and a buffer kept their laminar stre...