苯丙胺对大鼠海马突触素表达和突触结构的影响

Influence of amphetamine on synaptic structure and synaptophysin expression at hipprocampal formation in rats

目的探讨苯丙胺对大鼠海马突触素(SYN)蛋白质表达及突触结构的影响。方法将72只SD大鼠随机分为对照组、生理盐水组和苯丙胺组。用Western blotting检测大鼠海马结构内突触素表达的变化、用透射电镜观察大鼠海马CA3区突触的形态结构的变化。结果①苯丙胺组大鼠28d、42d的SYN相对表达量较对照组和生理盐水组相应时间明显降低,差异有统计学意义(P<0.05);苯丙胺组42d的SYN相对表达量较14d明显减少,差异有统计学意义(P<0.05)。②发现苯丙胺组大鼠较对照组的突触结构模糊、突触前后膜增厚,边界模糊,髓鞘出现松散分离样变。结论每天给大鼠肌注0.5mg/kg苯丙胺,28d出现海马结构突触素表达明显减少、海马CA3区突触结构明显损害;42d上述各项损害明显加重。

Objective To study the influence of amphetamine on synaptophysin(SYN) expression and synaptic srtructure at hippocampal formation in rats.Methods Seventy Sprague Dawley(SD) rats were randomly divided into three groups:control group、saline group and amphetamine group.The quantity of synaptophysin expression were studied between different phrases and groups by Western blotting.The morphologic change of synapse formation was observed by the transmission electron microscope.Results There was an obvious reduction of SYN expression for the amphetamine group in contrast to the control group and the saline group at 28 d and 42 d (P <0.05).And there was an obvious reduction of SYN expression at 42 d than 14 d in amphetamine group(P<0.05).The synaptic structure is unclear in amphetamine group.The presynaptic memebrane was thickener in amphetamine group than con trol group.Myelin sheath was loose and disrupted in amphetamine group.Conclusion Intramuscular injection of 0.5mg /kg amphetamine once everyday reduced expression of SYN and damaged the synaptic structure at CA3 sub-region in hippocampal formation.These damages were aggravated at 42 d.