多发性骨髓瘤患者骨髓间充质干细胞的生物学特性研究

Characterization and Functionality of Multiple Myeloma Patients Bone Marrow Mesenchymal Stem Cells

目的研究多发性骨髓瘤(MM)患者骨髓间充质干细胞(MSCs)的生物学特性及其体外支持造血的能力。方法取15例MM患者的骨髓MSCs,并在体外培养和扩增。观察细胞形态,应用流式细胞术检测MSCs免疫表型;体外诱导MSCs向骨和脂肪分化;RT-PCR检测MSCs造血因子表达情况;将MM骨髓MSCs作为滋养层,应用甲基纤维素半固体培养,检测其支持造血能力。结果 MM来源的MSCs形态为典型的纤维细胞样,该细胞群体表达CD29、CD44、CD105,而CD14、CD31、CD34、CD45、HLA—DR均表达阴性。油红O染色和VonKossa染色检测显示MM骨髓MSCs可以向骨和脂肪分化。MM骨髓MSCs表达造血相关因子,在体外可以维持和扩增造血干/祖细胞。结论 MM患者骨髓中存在具有多向分化能力的MSCs,其具有体外支持造血的功能。

Objective To study the characteristics of mesenthymal stem cells (MSCs) derived from multiple myeloma (MM) patients’ bone marrow in vitro.Methods Bone marrow mononuclear cells from MM were obtained,cultured and expanded.Immunophenotype and in vitro differentiation capacities were investigated.Expression of cytokines was detected by RT-PCR,and hematopoiesis support of MSCs in vitro was detected by long term bone marrow culture and the methylcellulose progenitor assay.Results MSCs derived from MM presented a typital fibrablast like morphology.They were positived for CD29,CD44 and CD105,while CD14,CD31,CD45,CD34,and HLA-DR were negative.Moreover,under suitable conditions,MM derived MSCs could be differentiated into osteocyte and adipocyte.At last,they expressed hematopoiesis cytokines and possessed ability of hematopoiesis support in vitro.Conclusion There is one cell population with characteristics of mesenchymal stem cells in MM bone marrow.They possess the ability of hematopoiesis support in vitro.