经脉配穴法选穴针刺对大鼠胃黏膜损伤修复的组织形态学影响

Histological Changes of Acute Gastric Mucosal Lesion Rats Treated by Different Acupoint Combination Groups on Meridians

目的:观察不同经脉配穴针刺对大鼠胃黏膜损伤修复的组织形态学影响。方法:以无水乙醇灌胃法制作急性胃黏膜损伤模型,采用肉眼观察、显微镜下观察及胃黏膜损伤指数(UI)计分等方法观察大鼠胃黏膜损伤修复的组织形态学变化。结果:模型组及各针刺组大鼠与空白组比较胃黏膜损伤指数升高,其中模型组、表里经配穴组、他经配穴组升高更明显(P<0.01或P<0.05);本经配穴组、表里经配穴组、同名经配穴组与模型组比较胃黏膜损伤指数显著降低(P<0.05),他经配穴组回降不明显。肉眼及显微镜观察大鼠胃黏膜,结果显示:本经配穴组、表里经配穴组、同名经配穴组胃黏膜色泽红润,略有少数点状出血,上皮基本完整,腺体排列较整齐,少量炎细胞浸润,胃黏膜无充血或充血较轻微。模型组及他经配穴组大鼠胃黏膜充血、水肿,可见点状出血和糜烂,上皮不完整,腺体受损,结构紊乱,细胞排列无序,胞核胞质模糊,毛细血管增生及出血,可见大量炎性细胞浸润。结论:本经配穴法、表里经配穴法、同名经配穴法针刺均可促进大鼠胃黏膜损伤的修复,他经配穴针刺作用不明显。

Objective:To observe histological changes of acute gastric mucosal lesion rats treated by different acupoint combination groups,and to analyze the protective effect of them.Methods:60 SD rats were divided randomly into 6 groups,10 rats each group.Group A was blank control group.Group B was model group.Rats in group C(combining points on the same meridians)was electroacupunctured in Zusanli and Chongyang.Rats in group D(combining points on the exteriorly-interiorly related meridians)was electroacupunctured in Zusanli and Taibai.Rats in group E(combining points on the meridians sharing the same name)was electroacupunctured in Zusanli and Hegu.Rats in group F(combining points on the unrelated meridians)was electroacupunctured in Chengjin and Jinggu.Acute gastric mucosal lesion rat model was built by ethanol ingestion.Gastric mucosal lesion index was recorded and histological changes of gastric mucosal tissues were observed under microscope.Results:①Compared with blank control group,UI was increased in other 5 groups,especially in group B,D,and F(P<0.05 or P<0.01).Compared with model group,UI was decreased in group C,D,E(P<0.05),no significant decrease in group F.②In group C,D,and E,gastric mucosa looked bloom,few punctuate hemorrhage,epithelium tissue was integrated,glandular organs was regular,few inflammatory cell infiltration,gastric mucosa was lightly congestive.In model group,and group F,gastric mucosa was congestive,dropsied,punctuate hemorrhage and erosion,epithelium tissue was incomplete,glandular organs was damaged and disorder,nucleocytoplasmic was fuzzy,capillary proliferation,Substantial inflammatory cell infiltration.Conclusion:3 kinds of point combination(acupoints of same Meridians,acupoints of Exteriorly-Interiorly Related Meridians,acupoints of Meridians Sharing the Same Name) have therapeutic effect.