针刺对眼压已控制的慢性高眼压兔视网膜保护作用的研究

The Protective Effect of Scupuncture Treatment on Retina in Rabbits Whose Intraocular Pressure was Reduced to Normal after Chronic Intraocular Pressure Elevated

目的:观察针刺对眼压已控制的慢性高眼压兔的保护作用。方法:以大耳白兔为实验动物,通过前房注射复方卡波姆溶液造成慢性高眼压模型,28天后通过滤过性手术使眼压恢复正常。分层随机分为模型组、针刺组、电针组、神经营养剂组,并设正常组。予针刺和电针治疗4周,观察其对各组兔视网膜形态学和视网膜节细胞(RGCs)计数的影响。结果:实验结束时各组动物出现显著差异,针刺组、电针组兔视网膜病理结构损伤较轻,视网膜节细胞(RGCs)数量明显高于模型组(P<0.05)。结论:针刺能保护高眼压损害的视神经,作用主要表现为减轻视网膜结构损伤,减少视网膜神经节细胞的凋亡。

Objective:To observe the protective effects of acupuncture treatment on the retina of the rabbits model whose intraocular pressure was reduced to normal after chronic intraocular pressure elevated(IOP).Methods:Elevated intraocular pressure was gotten by injection of 0.3 carbomer into anterior chamber of albino rabbit.IOP was reduced to normal through conventional glaucomatous trabeculetomy after the intraocular pressure continuously for 28 days.Acupuncture treatment was used to treat the rabbits everyday in the treated group after the trabeculetomy for 4 weeks.The pathologic changes were observed.A computer image analysis system was used to count the retinal ganglion cells(RGCs).Results:The microscopical results indicated that compared with experimental group retina swelled relatively gentler,and the degree of damaged retina ganglion cells in treatment group was lesser than that in experimental group.The number of RGCs of treatment group was more than the experimental group(P<0.05).Conclusion:Acupuncture treatment can protect the optic nerve from elevated IOP,by alleviated the damages of the retinal and preventing the apoptosis of RGCs.