电针对脑缺血再灌注大鼠缺血局部脑血管形成的影响

The Effect of Electro-acupuncture on Focal Cerebral Angiogenesis in Cerebral Ischemia-reperfusion Rats

目的:观察电针对脑缺血再灌注大鼠外周血EPCs数量和缺血脑皮层VEGFR-2和PECAM-1表达的影响,探讨电针促进脑缺血后血管形成的影响机制。方法:雄性SD大鼠随机分为正常组、假手术组、模型组、电针组。取"后三里"和"曲池"。流式细胞术检测外周血EPCs的数量,免疫组化观察缺血脑皮层VEGFR-2和PECAM-1的表达。结果:模型组在24 h EPCs数量达高峰,48 h开始降低;电针组EPCs数量24 h开始增加,48 h达高峰,72 h开始降低。模型组和电针组在24 h VEGFR-2和PE-CAM-1开始表达,表达随时间的延长而增加,电针组的表达较模型组增多。结论:电针能够提高脑缺血再灌注大鼠外周血EPCs数量,增加缺血脑皮层VEGFR-2、PECAM-1的表达,有利于促进缺血局部脑血管的形成。

Objective:To observe the effects of electro-acupuncture on the quantity of endothelial progenitor cells(EPCs) in peripheral blood and on the expression of Vessel Endothelium Growth Factor Receptor-2(VEGFR-2) and Platelet Endothelial Cell Adhesion Molecule-1(PECAM-1) in ischemic cerebral cortex of cerebral ischemia-reperfusion rats,discussing the mechanism of angiogenesis after cerebral ischemia with the effect of electro-acupuncture.Methods:Male rats were randomly and evenly assigned to normal group,sham-operation group,model group and EA group.'quchi' and 'housanli' were selected.EPCs quantity was displayed using flow cytometry.VEGFR-2 and PECAM-1 expression are observed with immunohistochemistry.Results:EPCs quantity in blood achieved peak in model group at 24hrs,and began to decreased after 48hrs;while in EA group EPCs quantity in blood started to increase at 24hrs,and reached peak in 48hrs,then decreased at 72hrs.VEGFR-2 and PECAM-1 expression began to increase at 24hrs in model group and EA group,and the expression increased with the time prolonged.The expression of electro-acupuncture group was more than that in model group.Conclusion:The electro-acupuncture could increase EPCs quantity in peripheral blood,and raise VEGFR-2 and PECAM-1 expression in ischemic cerebral cortex.Those can contribute to promote the formation of blood vessels in cerebral ischemia region.