眼针对局灶性脑缺血再灌注大鼠脑组织半暗区Caspase-8、Caspase-3 mRNA表达的影响

The Effect of Eye-acupuncture on the Expression of Caspase-3mRNA and Caspase-8mRNA in the Ischemic Penumbra

目的:探讨眼针疗法治疗缺血性脑血管病的机制。方法:线栓法制作大鼠局灶性脑缺血再灌注模型,分为假手术组、模型组与眼针组;取眼针上焦区、下焦区、肝区、肾区为施加因素;RT-PCR法检测Caspase-8、Caspase-3 mRNA表达。结果:眼针组与模型组比较,差异显著(P<0.01)。结论:眼针下调脑缺血再灌注缺血区脑组织Caspase-8、Caspase-3 mRNA组表达,从而抑制细胞凋亡,可能是眼针治疗缺血性脑血管病的机制之一。

Objective:The subject is to probe the possible mechanism of eye-acupuncture in the treatment of Ischemic Cerebrovascular Disease.Methods:Divided the rats into 3 groups by random,that is the sham-operated group,model group and eye-acupuncture group.Make the model rat of focal cerebral ischemia-reperfusion according to the improved method;to take on the Shangjiao area,Xiajiao area,liver area,kidney area for the imposition factors;Using RT-PCR method;test the expression of caspase-3mRNA and caspase-8mRNA.Results:Eye-acupuncture group compared with model group are significantly different(P<0.01).Conclusion:Eye-acupuncture may decrease the expression of Caspase-3 mRNA and Caspase-8mRNA in the ischemic penumbra to restrict the conduct of the apoptosis signals,This may be one of the mechanism of eye-acupuncture in the treatment of Ischemic Cerebrovascular Disease.