天灸关元、命门穴对佐剂性关节炎大鼠细胞免疫功能影响的比较研究

Comparative Research of the Influence ahout the Acu-point Ming Men and the Acu-point Guan Yuan Medicinal Vesiculation in Adjuvant Arthritis Rats

目的:分别观察天灸关元、命门穴对佐剂性关节炎大鼠细胞免疫功能的影响,探究关元、命门穴之间在细胞免疫调节方面的差异。方法:建立与类风湿性关节炎(RA)病理变化基本相似的佐剂性关节炎(AA)大鼠模型,随机分为空白对照组、模型组、天灸命门组、天灸关元组,测定血清中IL-2含量;计算胸、脾指数。观察天灸单穴命门、关元穴对关节炎症状的影响及免疫作用。结果:(1)对于IL-2:两穴均可升高IL-2,天灸关元、命门穴组间差异有统计学意义,天灸关元穴优于天灸命门穴组(P<0.05);(2)对胸、脾指数:天灸命门、关元穴均可提高胸指数,降低脾指数,但两组之间差异无统计学意义(P>0.05)。结论:天灸关元、命门穴均能提高AA大鼠IL-2水平,具有正向的免疫调节作用。天灸关元穴对AA大鼠细胞免疫功能的影响优于天灸命门穴。

Objective:To explore the influence of the acu-point Ming Men and the acu-point Guan Yuan medicinal vesiculation the cellular immunity in adjuvant arthritis rats.To explore the difference of the two groups in the cellular immunity in adjuvant.Methods:Rats model of adjuvant arthritis(AA) were made and randomized into control group,model group,model and medicinal Vesiculation the acu-point Ming Men group,model and medicinal Vesiculation the acu-point Guan Yuan group.The inhibitory effects on acute paw edema were observed and the paw thick and the levels of serum IL-2 levels were determined in experimental rats.Account the big rats Spleen,the thymus gland index number.To explore the influence of the acu-point Ming Men group,and the acu-point Guan Yuan medicinal vesiculation the cellular immunity in adjuvant arthritis Rats.Results:(1)The activities of IL-2 in the serum were significantly raised.The guan Yuan are better than the Min Men group in the affection of IL-2 and the arthritis index number.The differences have significant factors(P<0.05-0.0083).(2)The big rat the thymus gland index number were significantly raised.The big rat’s Spleen index number was significantly decreased.But the differences between the two Vesiculation groups have not significant factors.Conclusion:As far as the IL-2 in the serum are concerned,medicinal Ve-siculation of the acu-point Ming Men group,and the acu-points Guan Yuan have positive immunoregulation affection in AA rats.It may raise the activities of IL-2 in the serum.The guan Yuan are better than the Min Men group in the affection of IL-2.