两种芦荟多糖对小鼠肉瘤组织中凋亡相关蛋白bcl-2表达的影响

Effects of two kinds of Aloe polysaccharides on expression of apoptosis-related protein bcl-2 in sarcoma S_(180) tissues of mice

目的:探讨库拉索芦荟多糖和木立芦荟多糖对小鼠S180肉瘤组织中bcl-2蛋白表达的影响。方法:常规复制S180荷瘤小鼠模型,造模24h后,将荷瘤小鼠随机分为模型组、库拉索芦荟多糖组[25mg/(kg·d)]和木立芦荟多糖组[25mg/(kg·d)],模型组给予同容量生理盐水,连续灌胃(ig)给药10d后,处死小鼠取肉瘤组织,称取其重量并计算肿瘤抑制率,采用免疫组织化学方法检测肉瘤组织中bcl-2蛋白的表达。结果:库拉索芦荟多糖组与木立芦荟多糖组的肿瘤重量显著低于模型组,其肿瘤抑制率分别为42.53%和45.54%。两种芦荟多糖组肿瘤组织中bcl-2的表达显著低于模型组(P<0.05),而两种芦荟多糖组之间比较差异不显著。结论:库拉索芦荟多糖与木立芦荟多糖均可抑制小鼠移植性肿瘤S180的生长,其作用可能与下调凋亡相关蛋白bcl-2的表达,诱导肿瘤细胞凋亡有关。

Objective:To explore the effects of Aloe polysaccharides from A.barbadensis and A.arborescens on expression of apoptosis-related protein bcl-2 in sarcoma S180 tissues of mice.Methods:Models of sarcoma S180 bearing mice were made and divided into three groups,including model group,A.barbadensis group [25 mg/(kg·d)] and A.arborescens group [25 mg/(kg·d)].Two kinds of Aloe polysaccharides were administered orally for 10 days,all mice were sacrificed.Tumor weight was measured and tumor control rate was calculated,expression of bcl-2 protein in sarcoma S180 tissues was detected by immunohistochemistry.Results:Aloe polysaccharides from A.barbadensis and A.arborescens could evidently reduce the tumor weight of S180,the tumor control rate were 42.53% and 45.54% respectively.The expression of apoptosis-related protein bcl-2 treated with two kinds of APs was significantly lower compared with the model group (P<0.05),there was no remarkable difference between A.barbadensis group and A.arborescens group.Conclusion:Aloe polysaccharides from A.barbadensis and A.arborescens could inhibit sarcoma S180 growing,the pathogenesis is probably related with the downregulated expression of bcl-2 and inducing the apoptosis of tumor cells.