高效液相色谱-蒸发光散射检测法测定茶叶中单糖和双糖

Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography-Evaporative Light Scattering Detector

建立了高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定茶叶中单糖和双糖的检测方法。采用ShodexNH2P-50-4E(4.6mm×250mm,5μm)色谱柱,以水和乙腈为流动相进行梯度洗脱,梯度洗脱程序为:0～14min,85%乙腈;14～16min,85%～70%乙腈;16～28min,70%乙腈;28～32min,70%～85%乙腈,流速:1.0mL/min,柱温:20℃,雾化管温度为30℃,漂移管温度为70℃,氮气压力为310.275Pa。鼠李糖、阿拉伯糖、木糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖在3.202～184.828μg范围内程良好的线性关系,8种糖的加标回收率在90.97%～105.07%之间,最低检测限(S/N=3)范围在6.976～1376.297ng。该方法具有操作简便,分离效果好等特点。

A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH2P-50-4E(4.6 mm×250 mm,5μm) column was used at 20℃,acetonitrile and water used as mobile phase,for gradient elusion with the flow rate of 1.0 mL/min.The ratio of organic solvent was controlled by a gradient elution procedure.The procedure was as follows:85%acetonitrile in 0～14 min,70%～80%acetonitrile 14～16 min,70%acetonitrile 16～28 min,then 70%～80%acetonitrile 28～32 min.At last,the ratio of acetonitrile...