不同地区嗜人按蚊基因组DNA差异分析

Analysis of Diversities Among the Different Strains Anopheles anthropophagus Genomic DNA

[目的]为进一步确定我国嗜人按蚊是否存在不同的种型提供研究依据。[方法]通过随机引物多态性DNA(RAPD)技术获取四川、云南、江苏3个不同地区嗜人按蚊的DNA指纹图谱,比较嗜人按蚊不同地区基因组DNA的多态性,从分子水平上对不同分布区嗜人按蚊进行差异分析。[结果]不同地区的嗜人按蚊之间的DNA片段在绝大部分相同的情况下存在差异,3个不同地区基本上具有共同的扩增片段,反映了各地理区域基因组DNA的同源性;同时各个地区所特有的片段及条带亮度(扩增片段强度)又有差别。[结论]不同地区的嗜人按蚊在DNA水平绝大部分相同的前提下存在差异,这种差异的发现将为鉴定形态学上无法区分的嗜人按蚊地理种型提供分子水平的分类依据。

Aim: In order to provide the scientific basis for determinating furtherly whether there are different sub - species in Anopheles anthropophagus in China. Metliod: DNA fingerprint maps of Anopheles anthropophagus from Sichuan, Yunnan and Jiangsu provinces were drawn by using random amplified polymorphic DNA (RAPD) technique. Diversities among he different strains Anopheles anthropophagus were analyzed at genomic DNA level by identifying their DNA polymorphism. Results: In spite of the overwhelming likeness among DNA fragments of the three strains, there still existed some discrepancies. The common fragments indicated some degree homogeneity of the three strains genomes, their distinctive fragments and intensity suggested the diversity among the strains. Conclusion: At DNA level, althouth they were mostly alike, diversities among the different strains of Anopheles anthropophagus still existed, which can provide the scientific basis of molecule classification for identification of Anopheles anthropophagus geographical sub - species that can not be discriminated by morphology.