摘要
AIM To study the inhibitory effects of VES( RRR-α-tocopheryl Succinate, VES ), aderivative of natural Vitamin E, on benzo (a)pyrene (B (a) P)-induced forestomach tumor infemale mice.METHODS The model of B (a)P-inducedforestomach tumor was established according tothe methods of Wattenberg with slightmodifications. One hundred and eighty femalemice (6 weeks old) were divided into six groupsequally; negative control (Succinic acid),vehicle control ( Succinate + B (a) P), positivecontrol(B(a) P), high VES(2.5g/kg. b. w + B(a)P), Iow VES(1 .25 g/kg. b. w + B(a) P) ig as wellas VES by ip (20 mg/kg, b. w + B(a) P). Exceptthe negative control group, the mice wereadministrated with B(a)P ig. and correspondingtreatments for 4 weeks to study the anti-carcinogenetic effect of VES during the initiationperiod. The experiment lasted 29 weeks, inwhich the inhibitory effects of VES both ontumor incidence and tumor size were tested.RESULTS The models of B (a)P-inducedforestomach tumor in female mice wereestablished successfully. Some werecauliflower-like, others looked like papilla, evena few were formed into the ulcer cavities. VES at1.25 g/kg. b. w, 2.5 g/kg. b.w. by ig and 20 mg/kg. b. w. via ip could decrease the number oftumors per mouse (1.7 ± 0. 41, 1.6 ± 0.34 and 1.1±0.43), being lower than that of B(a)P group(5.4 ± 0.32, P＜0.05). The tumor incidence wasinhibited by 18.2%, 23.1% and 50.0%. VES at1.25g/kg.b.w., 2.5 g/ kg.b.w. by ig and20 mg/kg. b.w. via ip reduced the total volumeof tumors per mouse (54.8 ± 8.84, 28.4 ± 8.32and 23.9± 16.05), being significantly lower thanthat of B(a)P group (150.2±20.93, P＜0.01).The inhibitory rates were 63.5%, 81.1% and84.1%, respectively.CONCLUSION VES has inhibitory effects on B(a) P-induced forestomach carcinogenesis infemale mice, especially by ip and it may be apotential anti-cancer agent in vivo.
AIM To study the inhibitory effects of VES (RRR-α-tocopheryl Succinate, VES), a derivative of natural Vitamin E, on benzo(a) pyrene (B(a)P)-induced forestomach tumor in female mice. METHODS The model of B(a)P-induced forestomach tumor was established according to the methods of Wattenberg with slight modifications. One hundred and eighty female mice (6 weeks old) were divided into six groups equally; negative control (Succinic acid ), vehicle control (Succinate±B(a)P), positive control(B(a)P), high VES(2.5g/kg. b.w±B(a) P), low VES(1.25 g/kg.b.w±B(a)P) ig as well as VES by ip (20 mg/kg, b. w±B(a)P). Except the negative control group, the mice were administrated with B(a)P ig. and corresponding treatments for 4 weeks to study the anti- carcinogenetic effect of VES during the initiation period. The experiment lasted 29 weeks, in which the inhibitory effects of VES both on tumor incidence and tumor size were tested. RESULTS The models of B (a) P-induced forestomach tumor in female mice were established successfully. Some were cauliflower-like, others looked like papilla, even a few were formed into the ulcer cavities. VES at 1. 25g/kg. b. w, 2.5g/kg. b.w. by ig and 20mg/ kg. b. w. via ip could decrease the number of tumors per mouse(1.7±0.41, 1.6±0.34and 1.1 ±0.43), being lower than that of B(a)P group (5.4±0.32, P<0.05). The tumor incidence was inhibited by 18.2%, 23.1% and 50.0%. VES at 1.25 g/ kg.b.w., 2.5 g/ kg.b.w, by ig and 20 mg/kg, b.w. via ip reduced the total volume of tumors per mouse (54.8±8.84, 28.4±8.32 and 23.9±16.05), being significantly lower than that of B(a)P group (150.2±20.93, P<0.01). The inhibitory rates were 63.5%, 81.1% and 84.1%, respectively. CONCLUSION VES has inhibitory effects on B (a) P-induced forestomach carcinogenesis in female mice, especially by ip and it may be a potential anti-cancer agent in vivo.
基金
Project Supported by National Natural Science Foundation of China, No. 39870662
