摘要
AIM To study the abnormal expression of β-catenin gene and its relationship with invasiveness of primary hepatocellular carcinoma among Chinese people.METHODS Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, 4 normal liver tissues were immunohistochemically stained to study subcellular distribution of β-catenin. Semiquantitive analysis of expression of β-catenin gene exon 3 mRNA was examined by RT-PCR and in situ hybridization. The relationship between expressions of both β-catenin protein, mRNA and clinicopathological characteristics of HCC was also analyzed.RESULTS Immunohistochemistry showed that all normal liver tissues and para-cancerous tissues examined displayed membranous type staining for β-catenin protein,occasionally with weak expression in the cytoplasm.While 21 cases (61.8%) of HCC examined showed accumulated type in cytoplasms or nuclei. The accumuled type Labling Index (LI) of cancer tissue and paracancarous tissue was (59.9 ± 26.3) and (18.3 ± 9.7)respectively (P＜0.01). Higher accumulated type LI was closely related with invasiveness of HCC. Results of RTPCR showed the β-catenin gene exon 3 mRNA Expression Index (El) of 34 HCCs was higher than that of paracancerous tissue and normal liver tissue. Using in situ hybridization, the signal corresponding to β-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of para-cancerous and normal liver tissues. Over expression of β-catenin exon 3 was also found to be correlated with high metastatic potential of HCC.CONCLUSION Abnormal expression of β-catenin gene may contribute importantly to the invasiveness of HCC among Chinese people.
AIM To study the abnormal expression of β-catenin gene and its relationship with invasiveness of primary hepatocellular carcinoma among Chinese people. METHODS Thirty-four hepatocellular carcinoma(HCC) specimens and adjacent para-cancerous tissues,4 normal liver tissues were immunohistochemically stained to study subcellular distribution of β-catenin.Semiquantitive analysis of expression of β-catenin gene exon 3 mRNA was examined by RT-PCR and in situ hybridization.The relationship between expressions of both β-catenin protein,mRNA and clinicopathological characteristics of HCC was also analyzed. RFSULTS Immunohistochemistry showed that all normal liver tissues and para-cancerous tissues examined displayed membranous type staining for β-catenin protein, occasionally with weak expression in the cytoplasm, While 21 cases(61.8%)of HCC examined showed accumulated type in cytoplasms or nuclei.The accumuled type Labling Index(LI)of cancer tissue and para- cancarous tissue was(59.9 ± 26.3)and(18.3 ± 9.7) respectively (P<0.01).Higher accumulated type LI was closely related with invasiveness of HCC.Results of RT- PCR showed the β-catenin gene exon 3 mRNA Expression Index(El)of 34 HCCs was higher than that of para- cancerous tissue and normal liver tissue.Using in situ hybridization,the signal corresponding to β-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of para-cancerous and normal liver tissues.Over expression of β-catenin exon 3 was also found to be correlated with high metastatic potential of HCC. CONCLUSION Abnormal expression of β-catenin gene may contribute importantly to the invasiveness of HCC among Chinese people.
基金
National Ninth Five-year Plan of Medical Sciences of China(96-9064)105)
