摘要
AIM To analyze the association of HLA-DRBl with autoimmune hepatitis (AIH) in patients from China.``METHODS In .32 patients and 45 healthy controls,polymerase chain reaction amplification with sequencespecific primers (PCR-SSP) was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 with AIH.``RESULTS HLA-DRB1 typing by PCFLSSP showed that DR4had a significantly increased frequency among patients with AIH versus healthy control (46.9% versus 20.8%;relative risk 3.35, P=0.014). In subtypes of DR4, there was a trend of increase in the gene frequency of DRB10405 in patients with AIH versus healthy controls (21.9%vs 6.3%, P=0.04, but Pc 0.08). In addition, asignificant increase was found in the alleles frequency encoding QRRAA from the third hyperpolymorphic region of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P 0.016, Pc =0.028. RR 9.75).``CONCLUSION AIH in Chinese is associated with HLADR4. There is a relationship between QRRAA sequence within the third hyperpolymorphic region of the DRB allele and AIH in Chinese.
AIM To analyze the association of HLA-DRB1 with autoimmune hepatitis(AIH)in patients from China. METHODS In 32 patients and 48 healthy controls, polymerase chain reaction amplification with sequence- specific primers(PCR-SSP)was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 with AIH. RESULTS HLA-DRB1 typing by PCR-SSP showed that DR4 had a significantly increased frequency among patients with AIH versus healthy control(46.9% versus 20.8%; relative risk=3.35,P=0.014).In subtypes of DR4,there was a trend of increase in the gene frequency of DRB1~* 0405 in patients with AIH versus healthy controls(21.9% vs 6.3%,P=0.04,but P_c=0.08).In addition,a significant increase was found in the alleles frequency encoding QRRAA from the third hyperpolymorphic region of DR4 in the patients with AIH(86.7% of DR4 positive patients vs 40.0% in DR4 positive controls,P=0.016,P_c =0.028,RR=9.75). CONCLUSION AIH in Chinese is associated with HLA- DR4.There is a relationship between QRRAA sequence within the third hyperpolymorphic region of the DRB allele and AIH in Chinese.
