植物激素对水仙(Narcissus tazetta var.Chinensis)愈伤组织形成及分化的影响

Formation and Differentiation of Calli of the Effort of Plant Regulators on the Scale Propagation of the Chinese Sacred- lily(Narcissus tazetta var.Chinensis)

水仙鳞茎切块在附加不同种类和浓度的植物激素的MS培养基上可诱导产生白色或浅黄色的愈伤组织。形成的愈伤组织可以在上述新鲜培养基上不断继代培养。愈伤组织转至含BA或NAA的MS培养基上 ,均能产生分化不定芽。培养基中NAA浓度高有利不定根的分化。不定芽抽叶后 ,形成休眠的小鳞茎。在添加不同浓度的BA和NAA的MS培养基上 ,均能抽叶生根 ,由小鳞茎长成完整的植株。

The scale propagation of the Chinese sacred-lily could be induced to form white and yellow calli on the media with deferent plant regulators.The favorable medium is MS+BA1+2,4D0.1.The calli could differentiate on the media added BA and NAA.But the differentiation rate of roots could be increased, and the differentiation rate of buds decreased with increasing of NAA concentration in the media.The buds could grow up to small plants on the MS media with BA and NAA.The result can be used to obtain the vireo-free seedlings the Chinese sacred-lily.