金针茹组织分离研究

Study on the Tissue Isolation of Collybia Velutipes

对金针菇(ｃｏｌｌｙｂｉａ ｖｅｌｕｔｉｐｅｓ＜ＣＵＲＴ．ＥＸ ＦＲ＞)采用组织分离法制作菌种,割取菌柄与菌盖交际处,菌柄上部,菌柄中上部及菌柄中部的组织块在胡萝卜培养基,马铃薯培养基以及综合培养基中进行培养,实验结果得出菌柄中上部为最佳,菌柄中部亦较好,三种培养基中最佳配方为综合培养基。

Using tissue isolation method,the strain of Collybia Velutipes was got. Cut the tissue of the connection of thestipe and the pileus.the top of the stipe,the mid- top of the stipe and the middle of the stipe,and then cultivate them inthe carrot medium, the potato dextrose agar and the competitive medium. The result shows that the mid ?top tissue is thebest and the middle of the stipe is better. Among the three mediums, the comprehensive medium is the best optimum.