2008年绍兴地区腺病毒引起咽结膜热暴发流行病原学分析

Analysis on Shaoxing Area Pharyngeal Conjunctival Fever Virus Etiology Caused by Adenovirus in 2008

[目的]探讨2008年夏季发生于绍兴地区咽结膜热的病毒病原学。[方法]采用急性咽结膜炎患儿急性期咽及肛门拭子进行病毒分离,并同时用PCR法检查标本中病毒及分离病毒鉴定,对分离出部分毒株采用多个内切酶进行内切酶分析。此外,还测定患儿双份血中病毒的中和抗体增长。[结果]19份患儿咽拭子和肛门拭子病毒分离阳性率分别为63.2%;所有分离病毒株均为3型腺病毒。[结论]本次急性咽结膜炎暴发流行是由3型腺病毒引起。

[Objective] To investigate the summer of 2008 occurred in Shaoxing area pharyngeal conjunctival fever virus etiology.[Methods] Conjunctivitis in children with acute phase pharynx pharynx and anal swabs for virus isolation and at the same time PCR method used to check the virus samples and isolated the virus identified,isolated part of the strains used to carry out a number of enzyme endonuclease analysis.[Result]Children with double determination of the virus in the blood and antibodies growth.The results of 19 children with throat swab and anal swab positive for virus isolation rates were 63.2 percent;all isolated virus strains were of type 3 adenovirus.[Conclusions]The outbreak of acute conjunctivitis pharynx is caused by adenovirus type 3.