期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

α-乙酰乳酸脱羧酶基因在枯草芽孢杆菌中的整合型表达 被引量：7

Integrative Expression of α-acetolactate Decarboxylase Gene in Bacillus subtilis
下载PDF
导出
摘要 α-乙酰乳酸脱羧酶在啤酒生产中能加快啤酒成熟,有重要的应用价值。本研究将枯草芽孢杆菌启动子P43克隆到质粒pUC19-ALDC中的α-乙酰乳酸脱羧酶基因之前,得到重组质粒pUC19-P43-ALDC。重组质粒pUC19-P43-ALDC与质粒pMLK83-BN同源重组,筛选得到枯草芽孢杆菌整合质粒pMLK83-ALDC。用此整合质粒转化枯草芽孢杆菌1A751,挑选出新霉素抗性且无淀粉酶活性的重组菌株。此菌株用LB培养基在37℃、220r/min摇瓶培养过夜,测得α-乙酰乳酸脱羧酶活力为15.6U/mL,说明整合的α-乙酰乳酸脱羧酶基因能够在重组菌株中稳定传代和表达。本研究首次在枯草芽孢杆菌中用整合型的方式重组表达了α-乙酰乳酸脱羧酶,提出了一种有潜力的生产α-乙酰乳酸脱羧酶的新方法。 α-acetolactate decarboxylase plays an important applied role in speeding up the maturation of beer in beer production. In this study we obtained the recombinant plasmid pUC19-P43-ALDC by cloning promoter P43 of Bacillus subtilis in the front of α-acetolactate decarboxylase gene in plasmid pUC19-ALDC, which was ho-mologous recombinated with plasmid pMLK83-BN and obtaining the integration plasmid pMLK83-ALDC of Bacillus subtilis. Then we transformed the integration plasmid into Bacillus subtilis 1A751, and se...
作者 廖东庆 陈发忠 岳田芳 张云光 黄日波 李晓明
机构地区 广西南宁邦尔克生物技术有限责任公司 广西大学生命科学与技术学院 广西科学院
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2010年第5期843-848,共6页 Genomics and Applied Biology
基金 广西科学研究与技术开发计划项目(桂科产10100021-2)资助
关键词 Α-乙酰乳酸脱羧酶 枯草芽孢杆菌 整合表达 同源重组 α-acetolactate decarboxylase Bacillus subtilis Integrative expression Homologous recombination
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献18

  • 1石爱琴,胡海红,胡艳华,李敏,丁明,赵辅昆.青霉素酰化酶(PGA)在枯草芽孢杆菌基因组上的整合表达[J].浙江理工大学学报（自然科学版）,2009,26(5):776-781. 被引量：3
  • 2蒙健宗,李晓明,王青艳,卢福燊,周志强,李庆业,李丛,韦函忠,黄日波.高密度培养基因工程大肠杆菌生产α-乙酰乳酸脱羧酶[J].广西科学,2001,8(4):284-286. 被引量：10
  • 3Kay Terpe.Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems[J]. Applied Microbiology and Biotechnology . 2006 (2)
  • 4Cai Heng,Zhongjun Chen,Lianxiang Du,Fuping Lu.Expression and Secretion of an Acid-Stable α-Amylase Gene in Bacillus Subtilis by SacB Promoter and Signal Peptide[J]. Biotechnology Letters . 2005 (21)
  • 5Anne Sietske,B?rge Diderichsen.On the safety of Bacillus subtilis and B. amyloliquefaciens: a review[J]. Applied Microbiology and Biotechnology . 1991 (1)
  • 6Sven Erik Godtfredsen,Martin Ottesen.Maturation of beer with α-acetolactate decarboxylase[J]. Carlsberg Research Communications . 1982 (2)
  • 7Gat O.,Inbar I,Aloni-Grinstein R,Zahavy E,Kronman C,Mendelson I,Cohen S,Volan B,Shafferman A.Use of a promoter trap system in Bacillus anthracisand Bacillus subtilis for the development of recombinantprotective antigen-based vaccines. Infection and Immunity . 2003
  • 8Bubeck, P,Winkler, M,& Bautsch, W.Rapid cloning by homologous recombination in vivo. Nucleic Acids Research . 1993
  • 9Gruss A,Ehrlich SD.The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiological Reviews . 1989
  • 10Sambrook J,Fritsch EF,Maniatis T.Molecular Cloning: A Laboratory Manual. . 1989

二级参考文献18

  • 1Brockmeier U, Wendorff M, Eggert T. Versatile expression and secretion vectors for bacillus subtilis[J]. Current Microbiology, 2005, 52(5):143--148.
  • 2Gruss A, Ehrlich S D. The family of highly interrelated singlestranded doexoyribonucleic acid plasmids[J]. Microbiol, 1989, 53(2): 231--241.
  • 3Janniere L, Bruand C, Ehrlich S D. Structurally stable bacillus subtilis cloning vectors[J]. Gene, 1990, 87(1): 53--61.
  • 4Duncan C H, Wilson G A, Young F E. Mechanism of integrating foreign DNA during transformation of bacillus subtilis [J]. Proc Natl Acad Sci USA, 1978, 75(8): 3664--3668.
  • 5Yang S, Huang H, Zhang R, et al. Expression and purification of extracetlular penicillin G acylase in bacillus subtilis[J]. Protein Exp Purif, 2000, 21(1): 60--64.
  • 6Spizizen J. Transformation of biochemically deficient strains of bacillus subtilis by deoxyribonucleate[J]. Proc Natl Acad Sci USA, 1958, 44(10): 1072--1078.
  • 7Shanghai Third Pharmacial Factory. Method to determine penicillin acylase fast and conveniently[J]. Med Ind, 1978, 7.. 22--24.
  • 8Eppelmann K, Doekel S, Marahiel M A. Engineered biosynthesis of the peptide antibiotic bacitracin in the surrogate host bacillus subtilis[J]. Biological Chemistry, 2001, 276(37): 34824--34831.
  • 9Bhavsar A, Zhao X, Brown E. Development and characterization of a xylose-dependent system for expression of cloned genes in bacillus subtilis: conditional complementation of a teichoic acid mutant[J]. Applied and Environmental Microbiology, 2001, 67:403--410.
  • 10Hartl B, Wehrl W, Wiegert T, et al. Development of a new integration site within the bacillus subtilis chromosome and construction of compatible expression cassettes[J]. Bacteriology, 2001, 8(183): 2696--2699.

共引文献11

同被引文献74

引证文献7

二级引证文献8

基因组学与应用生物学
2010年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部