摘要
为研究猪精子获能过程中蛋白酪氨酸磷酸化的变化规律,将猪精子悬浮于改良的Tris缓冲液(mTBM)获能培养基中,在5%CO2孵箱37℃培养,以考马斯亮蓝染液染色为指标评价精子获能状态,用免疫荧光技术检测酪氨酸磷酸化的蛋白在精子上的分布。结果显示,随着获能的进行,发生蛋白酪氨酸磷酸化的精子占总精子的百分比增加,由未获能前35%增至获能1.5 h时的88%,酪氨酸磷酸化的蛋白分布变广,由精子头部扩增至精子头部、鞭毛主段和中段。
The aim of the present study was to detect the localization and level of tyrosine phosphorylated proteins during in vitro capacitation of pig sperms.Sperm from mature pigs were incubated in modified mTBM under 5% CO2 in air at 37 ℃.The capacitation effect was assessed by Coomassie brilliant blue staining.Localization of tyrosine phosphorylation was checked by indirect immunofluorescence assay.The results showed that pig sperms were a time-dependent increase in protein tyrosine phosphorylation during the in ...
出处
《畜牧与兽医》
北大核心
2010年第9期20-23,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30960250)
关键词
精子获能
酪氨酸磷酸化
猪
capacitation
tyrosine phosphorylation
pig
