膜分离法纯化浓缩猪伪狂犬病疫苗毒的效果

Purification and concentration effects of swine pseudorabies vaccine virus by membrane separation

本研究使用不同孔径的陶瓷(有机)膜过滤器,对不合格的猪伪狂犬病毒细胞收获液(病毒含量≤104TCID50/mL)滤除杂蛋白、超滤浓缩、除菌处理得到纯化浓缩的猪伪狂犬病疫苗病毒液;然后对纯化浓缩的猪伪狂犬病疫苗病毒液分别进行杂蛋白去除率检验与无菌检验、病毒含量测定、安全检验、效力检验;将检验合格的纯化浓缩的猪伪狂犬病疫苗病毒液添加保护剂冻干,并对纯化浓缩的猪伪狂犬病冻干活疫苗进行以上各项检验,以及进行免疫猪体内抗体消长变化的检测。结果表明:纯化的猪伪狂犬病疫苗杂蛋白去除率平均达到68.3%以上,病毒含量≥105TCID50/mL,效力检验合格;免疫猪体内抗猪伪狂犬病毒抗体增长幅度比同时期未纯化的常规疫苗显著,其中免疫至84 d时中和抗体效价平均高达40.35稀释倍数左右,比常规疫苗中和抗体效价平均高出14.22稀释倍数。此项研究为畜禽疫苗的纯化提供一定的参考。

Different aperture ceramics(organic) membrane filters were used to purify and concentrate the unqualified swine pseudorabies virus(PRV) cultures(virus content ≤ 104 TCID50/mL).And then a series of tests were conducted on the concentrated vaccine virus,including impurity protein removal test,sterility test,determination of virus content,security and effectiveness tests.The vaccine virus which was demonstrated to be qualified was lyophilized in protection agent.The above tests were conducted on the lyophilize...