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Modification of Cre Gene by PCR Method

Modification of Cre Gene by PCR Method
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摘要 Cre/LoxP site-specified recombination system is mainly used for excision,inversion and integration of target gene.Therefore,this system can be used for plant marker free genetic transformation,site-specific transgene expression and so on.However,the application of this system was limited due to its low expression and excision efficiency.In this study,an intron,which can enhance gene expression in plants,was inserted into Cre by using PCR method.And a modified Cre gene,named Crein,was obtained.This gene was ... Cre/LoxP site-specified recombination system is mainly used for excision,inversion and integration of target gene.Therefore,this system can be used for plant marker free genetic transformation,site-specific transgene expression and so on.However,the application of this system was limited due to its low expression and excision efficiency.In this study,an intron,which can enhance gene expression in plants,was inserted into Cre by using PCR method.And a modified Cre gene,named Crein,was obtained.This gene was ...
作者 WANG Yong,LUO Hong,XU Yaying,LV Tao,and CHEN Dian College of Horticulture,Northeast Agricultural University,Harbin 150030,China
出处 《Journal of Northeast Agricultural University(English Edition)》 CAS 2010年第2期27-31,共5页 东北农业大学学报（英文版）
基金 Supported by Project of the Education Department of Heilongjiang Province (11511035) Dr Start-up Found Research of Northeast Agricultural University
关键词 CRE recombinase INTRON PCR MODIFICATION CRE recombinase intron PCR modification
分类号 Q78 [生物学—分子生物学]
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参考文献5

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共引文献40

Journal of Northeast Agricultural University(English Edition)
2010年 第2期

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