小麦蓝矮病植原体FrvX基因的克隆及致病性测定

Cloning and pathogenicity analysis of endo-1,4-beta-glucanase gene (FrvX) from wheat blue dwarf phytoplasma

【目的】克隆小麦蓝矮病(WBD)植原体β-1,4-内切葡聚糖酶基因(FrvX),验证其在植原体侵染寄主中的作用,为更好地防控小麦蓝矮病奠定理论基础。【方法】采用PCR技术从小麦WBD植原体中扩增到FrvX全基因,将其插入到病毒载体pgR107中,构建重组病毒载体pgR107-FrvX,用农杆菌介导法侵染本氏烟草,观察烟草表型的变化。【结果】FrvX基因全长1071bp,编码356个氨基酸。重组病毒载体接种烟草22d后,有9株烟草植株表现出明显的花叶、黄化症状,RT-PCR检测到发病植株接种7d后的叶片有FrvX基因的转录。【结论】FrvX基因接种烟草导致烟草表型发生变化,推测其与植原体病害的发生有关。

【Objective】In order to effectively control the wheat blue dwarf(WBD)phytoplasma and identify the WBD phytoplasma has the similarities to known phytoplasma virulence factors,we cloned the endo-1,4-beta-glucanase(FrvX)gene from WBD.【Method】We amplified the endo-1,4-beta-glucanase(FrvX) gene from WBD by polymerase chain reaction,then inserted the FrvXgene to double expression virus vector pgR107 and inoculated tobacco through agrobacterium to observe symptoms manifested.【Result】The FrvXgene from WBD phytoplasm...