秀丽线虫缺氧相关剪接调节因子的筛查

Screening of the hypoxia-related splicing regulatory factors of C.elegans

【目的】基于RNA干涉文库,寻找与秀丽线虫缺氧损伤表型相关的剪接调节因子。【方法】利用喂饲RNA干涉(RNA interference,RNAi)文库筛选技术,对秀丽线虫基因组中的351个RNA结合蛋白(RNA bindingprotein,RBP)基因进行筛选。【结果】经过2次筛选共得到5个与缺氧相关的RBP编码基因,其中剪接因子1个,参与mRNA翻译过程的基因4个。【结论】剪接调节因子可能在mRNA的剪接和翻译水平上参与低氧应答。

【Objective】The study was to find the splicing regulatory factors related to hypoxic injury phenotype.【Method】RNA-mediated interference(RNAi)library was used to target 351 RNA binding protein(RBP)-coding genes of C.elegans.【Result】RNAi knockdown of 5 hypoxia-related RBP genes was obtained,with a splicing factor and 4 genes involved in mRNA translation process.【Conclusion】Splicing regulatory factors may participate in the process of mRNA splicing and translation in hypoxic response.