宁夏水稻高效再生体系的建立

Efficient regeneration system from in vitro of rice in Ningxia

【目的】建立宁夏水稻高效转化再生系统,为利用基因工程技术定向改良宁夏水稻品种奠定基础。【方法】以8个宁夏主栽水稻品种成熟胚为外植体,利用组织培养法,研究了基因型、外源激素、头孢霉素(Cef)对外植体培养及再生的影响。【结果】在愈伤组织诱导培养基中添加ABA 2 mg/L及在分化培养基中添加6-BA 3 mg/L+NAA 0.5 mg/L,对愈伤组织诱导、分化及出苗有显著的促进作用;在此优化条件下,秋田小町再生情况最好,其诱导率和出苗率分别达100.00%和300%;优育27号次之,分别达95.00%和286.7%。此外,初步确定转化时Cef的质量浓度上限为500 mg/L。【结论】明确了外源激素、基因型及Cef对宁夏水稻成熟胚离体培养的影响效果,初步建立了宁夏水稻高效转化再生系统。

【Objective】 In this study,a highly efficient regeneration system from mature embryo of rice in local varieties was established preliminarily for genetic transformation.【Method】 Using mature embryo of eight rice varieties in Ningxia as explants and utilizing the plant tissue culture method,the effect of exogenous hormone,genotype and cephamycin(Cef) on in vitro culture and regeneration was studied.【Result】 The result showed that the supplementation of 2 mg/L of ABA in inducing medium expressed the highest ef...