金黄节杆菌DR-536分泌新型溶栓酶FA-Ⅰ发酵条件的优化

Optimization on fermentation of Arthrobacter aurescens strain DR-536 to secrete a novel fibrinolytic enzyme FA-Ⅰ

【目的】对金黄节杆菌DR-536(Arthrobacter aurescens strain DR-536)分泌产生新型溶栓酶FA-Ⅰ的发酵条件进行优化。【方法】通过纤维蛋白平板法,测定不同氮源、碳源、复配氮源、金属离子、NaCl、初始pH、接种量、装液量、发酵时间和培养温度条件下菌株DR-536分泌的溶栓酶产量。【结果】菌株DR-536(A.aurescens strain DR-536)分泌产生溶栓酶FA-Ⅰ的最佳发酵培养基配方为:黄豆粉1.0%,酵母膏1.0%,NaCl2.0%,FeSO40.05%,pH6.0。最佳发酵条件为:接种量4%,装液量20mL/150mL,发酵时间72h,发酵温度30℃。【结论】金黄节杆菌DR-536(A.aurescens strain DR-536)在普通培养条件下能够大量分泌溶栓酶FA-Ⅰ,适于大规模发酵,便于该酶的开发利用。

【Objective】The fermentation conditions of Arthrobacteraurescensstrain DR-536 to secrete a novel fibrinolytic enzyme FA-Ⅰ were optimized.【Method】Through the fibrin plate method,the productivity of fibrinolytic enzyme FA-Ⅰ secreted by strain DR-536under different nitrogen sources,carbon sources,combined nitrogen sources,metal ions,NaCl,initial pH,inoculation volume,medium volume,fermentation time and temperature was measured.【Result】The optimal components of medium for strain DR-536 secreting FA-Ⅰwere as foll...