反相高效液相色谱法分析化学合成胸腺素α_1

Analysis of Chemical Synthetic Thymosin α_1 by Reversed-phase High Performance Liquid Chromatography

利用反相高效液相色谱 (RP HPLC)法 ,对化学合成的胸腺素α1进行了分析。采用PLATINUMC18柱 (2 5 0mm× 4 .6mmi.d .,5 μm ,10nm) ,柱温 :室温 ;流动相A :10 0 %H2 O(含 0 .1%TFA) ;B :90 %乙腈水 (含0 .1%TFA) ,18%B A的等梯度洗脱 ;流速为 1mL/min ;检测波长为 2 14nm ;进样量为 2 0 μL。用外标法定量。实验结果表明 ,该测定方法的检出限为 4mg/L ;平均回收率为 95 .8% ;日内精密度 (用相对标准偏差RSD表示 )均在 10 %以内 ,胸腺素α1在选定的浓度范围内具有良好的线性相关性。该方法简便、快速、准确 ,2 0min内就可以完成一个样品的分析 ,故对胸腺素α1的定量分析具有重要的实用价值。

A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of chemical synthetic thymosin α_1 under a standardized common condition. In the method thymosin α_1 was chromatographically analyzed on a PLATINUM C_ 18 column(250 mm×4.6 mm i.d.,5 μm,10 nm)within 20 min,using a mobile phase A: 100% water (containing 0.1% trifluoroacetic acid (TFA)) and B: 90% acetonitrile/water (containing 0.1% TFA) at the ambient temperature with a flow rate of 1.0 mL/min . The detection wavelength was 214 nm. The good linear relationship was obtained under the chromatographic condition. The detection limit was 4 mg/L. The average recovery was 95.8%. The RSDs of intra-day were all less than 10%. This method is simple,fast,sensitive,and suitable for the analysis of thymosin α_1.