燃煤型氟中毒大鼠血清睾酮水平的变化及机制的初步研究

Preliminary study on effects and mechanism of coal-burning fluorosis in rats' serum testosterone.

目的研究燃煤型氟中毒大鼠血清睾酮的变化及可能的机制。方法以SD大鼠为研究对象,按体质量均衡随机分为对照组、低氟组、中氟组、高氟组4组。各染毒组喂饲含不同比例的燃煤型氟中毒病区煤烘玉米的饲料,复制燃煤型氟中毒动物模型。180d以股动脉放血法处死动物,查看氟斑牙,测尿氟、血清睾酮水平,常规制成肾上腺和睾丸HE染色病理切片和睾丸电镜超薄切片,观察肾上腺网状带和睾丸间质细胞结构的变化。结果建成氟中毒动物模型。与对照组相比,各染毒组大鼠血清睾酮水平均降低(P<0.01)。随氟剂量增加,睾酮水平降低更加明显(P<0.01)。光镜下观察,肾上腺网状带和睾丸间质细胞无明显损伤;透射电镜观察,睾丸间质细胞损伤明显,胞浆出现大量脂褐素颗粒,甚至细胞核固缩、溶解,细胞破裂。结论燃煤型氟中毒大鼠血清睾酮降低,睾丸间质细胞的损伤是其主要原因。

Objective To preliminary study the effects and mechanism of coal-burning fluorosis on testosterone level in serum of rats.Methods SD rats taken as the experimental objects were randomly assorted into 4 groups which were control group,low-dose fluoride group,middle-dose fluoride group,and high-dose fluoride group.The exposed groups were fed with the fodder with different proportions of corn dried by burning coal coming from endemic fluorosis areas,to establish the animal model of coal-burning fluorosis.All r...