家蚕、野桑蚕海藻糖酶基因启动子的特性及其滞育激素的转录调节

Characterization of Trehalase Gene Promoters From Silkworm, Bombyx mori and Bombyx mandarina and Transcriptional Regulatory Effects of Diapause Hormone on Them

以pGEM 3Z为载体 ,分别构建了家蚕 (Bombyxmori)和野桑蚕 (Bombyxmandarina)海藻糖酶基因启动子控制的荧光素酶基因报告质粒 ,利用昆虫细胞瞬时表达系统进行启动子特性分析 ,并就家蚕滞育激素对海藻糖酶基因启动子转录活性的调节作用进行了研究。结果表明 :家蚕和野桑蚕海藻糖酶基因启动子在家蚕Bm5细胞、Sf2 1细胞中都有转录活性 ,但是活性都很低 ;家蚕滞育激素在体外条件下对家蚕海藻糖酶基因启动子活性的调节有明显的剂量效应 ,13～ 33nmol/L时有明显的增强作用 ,由此推测来自家蚕卵巢的Bm5细胞可能存在与滞育激素作用的受体。

By using pEGM-3Z as vectors,the reporter plasmids were constructed with a luciferase gene under the control of trehalase gene promoters from Bombyx mori and Bombyx mandarina respectively for transient expression in insect cell lines.Meanwhile,the regulatory effect of diapause hormone(DH) on the transcriptional activity of trehalase gene promoter was studied.Both trehalase gene promoters from B.mori and B.mandarina showed low but significantly transcriptional activities in Bm5 cell line and Sf21 cell line.The artificial synthesized DH of Bombyx mori revealed a typical dose-dependent pattern on trehalase gene promoter activity in vitro, i.e.in the concentration of 13～33 nmol/L DH enhanced the promoter activity signifiantly.Thus we deduce that there are possibly DH receptors in the ovary-derived Bm5 cell line.