两个甘蓝自交系自交不亲和S单元型的初步鉴定

Preliminary Identification of S Haplotypes of Two Self-incompatible Lines in Cabbage(Brassica oleracea var. capitata L.)

根据甘蓝SRK基因序列保守区设计特异性引物,通过PCR扩增和克隆测序,结合生物信息学分析方法对两个甘蓝自交系材料的S单元型进行初步鉴定。BLAST分析表明:甘蓝自交系96-100与Brassica oleracea的SRK29基因核苷酸序列相似性最高(91%),但蛋白序列与S14相似性最高(在84%的序列覆盖度下相似性达100%)。自交系俄引165与Brassica oleracea的BoSRK28基因核苷酸序列相似性达100%,推测其S单元型为S28。

According to the conserved sequence of S locus kinase(SRK)gene in cabbage(Brassica oleracea var.capitata L.),a pair of SRK-specific primers was designed.The S haplotypes of 2 inbred lines in cabbage were identified by PCR amplification and DNA sequencing,combined with bio-informatics analysis.BLAST results indicated that the self-incompatible line 96-100 showed 91 % similarity in nucleotide sequence with SRK29 gene in cabbage,and 100 % similarity under 84 % query coverage of protein residue with S14 haploty...