用选择性培养基检测辣椒种子疫霉菌

Detection of Phytophthora Capsici from Pepper Seeds by Selective Medium

在国内外疫霉菌选择性培养基配方研究的基础上,配制含有多菌灵的选择性培养基(匹马霉素10mg、氨苄青霉素250mg、利福平10mg、五氯硝基苯100mg、恶霉灵100mg、多菌灵10mg),能有效地抑制镰孢菌等杂菌的生长。用此培养基成功地从贮存1、10、30、60、90、120d的辣椒疫病种子上分离出疫霉菌,贮存120d时分离率为2%。

A selective medium contained Pimaricin 10 mg,Ampicillin 250 mg,Rifampicin 10 mg,PCNB 100 mg,Hymexazol 100 mg,Carbendazim 10 mg can effectively inhibit the development of Fusarium sp.et al.In this paper,Phytophthora capsici was successfully isolated from pepper seeds that had been kept for 1,10,30,60,90,120 days with the selective medium,and the separation rate was 2 % on the 120th.