小菘菜游离小孢子培养技术研究

Studies on Isolated Microspore Culture in〔Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee〕

以4个引自日本的小菘菜杂交种为试材进行小孢子培养,对影响小菘菜胚状体发生及其成苗的因素进行分析。结果表明:通过游离小孢子培养,获得了大量的小孢子胚状体及再生植株85株;不同基因型间的小孢子胚诱导率差异显著;4℃低温预处理24h对小孢子胚的发生具有促进作用;NLN-13+0.2mg·L-16-BA+0.1mg·L-1NAA为适宜的诱胚培养基;50r·min-1低频振荡培养有利于提高子叶形胚发生的比例;MS+3%蔗糖+0.75%琼脂+0.1g·L-1活性炭是小孢子植株继代和壮苗的适宜培养基;MS+3%蔗糖+0.75%琼脂+0.1mg·L-1NAA是小孢子植株适宜的生根培养基。

This study is to establish a stable and efficient system of isolated microspore culture in〔Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee〕.The key factors influencing embryogenesis and plant regeneration were studied with 4 hybrid varieties introduced from Japan.Embryoids and 85 regenerated plants were obtained.Results showed that genotypes had extremely significant effect on frequency of embryogenesis.Low temperature pretreatment at 4 ℃ for 24 h could enhance embryo frequencies.NLN-...