摘要
目的应用超声造影评价大鼠肾脏冷缺血-再灌注损伤皮质血流灌注参数的变化及其与病理损伤的相关性,探讨静脉连续输注法超声造影评价肾血流灌注的价值。方法雄性SD大鼠随机分为2组,假手术组(15只)仅切除右肾,冷缺血-再灌注损伤组(15只)建立肾脏冷缺血-再灌注损伤模型,分别于术后第1、3、7天取5只大鼠行肾脏超声造影,定量测定肾皮质峰值声学强度(A)、造影剂灌注速率(β)、血流量(A×β)。统计分析肾皮质血流参数的变化及其与病理损伤的相关性。结果假手术组与冷缺血-再灌注损伤组大鼠同组之间不同时间点、两组之间对应时间点之间比较血流参数A值均无显著差异(P(0.05);冷缺血-再灌注损伤组术后第1天β值、A×β值降低,与假手术组比较差异有统计学意义(P<0.05,P<0.01)。冷缺血-再灌注损伤组术后第3天β值、A×β值开始改善,但仍显著低于假手术组(P(0.01);术后第7天β值、A×β值明显改善,与假手术组比较无显著差异(P(0.05)。经统计相关分析,肾小管坏死积分与A值无显著相关性(r=-0.43,P(0.05);与β值、A×β值均成显著负相关(r=-0.75、-0.77,P<0.01)。结论大鼠肾脏冷缺血-再灌注损伤皮质血流减少主要是由于血流灌注速度的降低所致;静脉连续输注法超声造影可定量监测肾皮质血流的变化。
Objective The aim of this study was to investigate the abnormal renal cortical haemodynamics and its correlation with nephropathy in rat models with cold ischemia-reperfusion injuries(CIRI),and to evaluate the value of continuousinfusion contrast-enhanced ultrasound (CEUS) in monitoring the anomalies of renal haemodynamics.Methods Thirty-four male Sprague-Dawley rats were randomly divided into two groups.Fifteen rats in sham-surgery group (SSG) were removed with their right kidneys only,and the other fiftee...
出处
《中华医学超声杂志（电子版）》
2011年第5期950-957,共8页
Chinese Journal of Medical Ultrasound(Electronic Edition)
关键词
超声检查
造影剂
肾
再灌注损伤
Ultrasonography
Contrast media
Renal
Reperfusion injury
