不同SDS-PAGE分离胶浓度条件下大豆贮藏蛋白亚基的分辨效果

The resolving effect of soybean storage protein subunits under different separation gel concentrations of SDS-PAGE

采用SDS-PAGE浓缩胶浓度为5%,分离胶浓度分别为9%、10%、11%、12%、13%的凝胶系统,探讨不同分离胶浓度条件下大豆籽粒主要贮藏蛋白(7S和11S组分)亚基的分辨效果。结果表明,分离胶浓度大小对大豆贮藏蛋白(7S和11S组分)亚基电泳图谱分辨率的影响非常明显。分离胶浓度为9%和10%时,7S组分亚基(α′、α和β)分辨效果较好;分离胶浓度为13%时,11S组分各亚基(A3、A4A2、A1aA1b、A5、B3、B1aB1bB2和B4)分辨效果较好。如需获得较好的7S和11组分亚基综合分辨效果,以分离胶浓度为12%的凝胶系统为佳。

The experiment attempt to investigate the resolving effect of soybean major storage protein (7S and 11S fraction) subunits under different separation gel concentrations of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the resolving effect of soybean major storage protein subunits could be greatly affected by the concentration change of resolving gel. The good resolving effect of 7S fraction subunits (α′、α and β) of soybean storage protein could be obtained when the resolving gel concentrations were 9% and 10%, and the good resolving effect of 11S fraction subunits (A3、A4A2、A(1a)A(1b)、A5、B3、B(1a)B(1b)B2 and B4) of soybean storage protein could be obtained when the resolving gel concentrations were 13%. Furthermore, 12% resolving gel concentration should be the best choice to obtain the good resolving effect of 11S and 7S fraction subunits.