吉兰-巴雷综合征相关空肠弯曲菌waaF基因序列对比研究

Comparison of waaF gene of Guillain-Barré Syndrome-associated Campylobacter jejuni strains

目的了解吉兰-巴雷综合征(GBS)相关空肠弯曲菌(C.jejuni)waaF基因核酸及氨基酸序列特征,分析其遗传进化关系。方法选取3株致GBS C.jejuni菌株,提取其基因组测序。应用生物信息学软件,以NCTC11168序列为参照进行比对,分析waaF基因碱基及相应氨基酸突变、二级结构变化,计算遗传距离。结果 3株致GBS C.jejuni waaF基因均由960个碱基构成,相同碱基突变位点有5个,1081462位zhanxingT→A,lulei、qiaoyuntaoT→G导致相同氨基酸改变:185位D→E,该位点导致α螺旋结构的改变。3株致GBS C.jejuni waaF基因遗传距离较小。结论 GBS相关C.jejuni菌株waaF基因序列存在相同碱基及氨基酸突变及二级结构变化,这可能与C.jejuni致GBS能力的改变相关。河北地区致GBS的C.jejuni在进化上存在聚类现象。

Objective To investigate the pathogenic mechanism of Guillain-Barré Syndrome(GBS)-associated Campylobacter jejuni(C.jejuni) and the phylogenic relationship of them.Methods 3 GBS-associated C.jejuni strains were cultured to get genome sequences,the 3 waaF sequences were compared with the NCTC11168 to find the base and amino acid mutations,the change of secondary structure and phylogenic relationship with bioinformatics software.Results The waaF gene of 3 GBS-associated C.jejuni strains all had 960 base pairs...