摘要
目的探讨高血压对内皮祖细胞粘附、迁移功能及再内皮化能力的影响。方法选择初发的原发性高血压患者和性别、年龄匹配的健康志愿者各10例,分离其外周血的单个核细胞诱导分化成内皮祖细胞,并通过免疫荧光标记等方法进行形态学观察和鉴定。培养7天后分别观察2组内皮祖细胞的粘附和迁移能力。建立裸鼠颈动脉内皮损伤模型,分别移植健康志愿者与高血压患者的内皮祖细胞,观察损伤血管再内皮化情况。结果外周血单个核细胞在体外诱导分化成为内皮祖细胞,ac-LDL吞噬及lectin抗体荧光标记双阳性。高血压患者组内皮祖细胞的粘附与迁移能力均明显低于健康志愿者组,裸鼠颈动脉内皮损伤修复面积亦小于健康志愿者组。结论高血压患者循环内皮祖细胞粘附、迁移功能及再内皮化能力的下降,提示内源性血管修复能力下降可能是高血压血管内皮损伤的重要机制。
Objective To observe the effects of hypertension on the migration, adhesion and reendothelialization capacities of endothelial progenitor cells(EPCs).Methods Ten patients with hypertension and 10 age and sex-matched healthy subjects without other cardiovascular risk factors were enrolled into the study. The peripheral venous blood mononuclear cells from the subjects were inducted into EPCs, which were morphologically observed and identified by fluorescent immunological labeling. EPCs migration, adhesion and...
出处
《中国分子心脏病学杂志》
CAS
2011年第1期23-25,共3页
Molecular Cardiology of China
基金
国家自然科学基金重点项目(u0732002)
关键词
高血压
内皮祖细胞
内皮
再内皮化
Hypertension
endothelial progenitor cells
endothelium
reendothelialization
