葛藤与葛根抗炎、耐缺氧作用对比研究及其安全性评价

Comparative study on anti-inflammation and anti-hypoxia activity between Pueraiae lobatae caulis and Pueraiae lobatae radix and the security assessment of Pueraiae lobatae caulis

目的:与葛根对比,初步探讨葛藤抗炎、耐缺氧作用,并对葛藤的安全性进行评价,为葛藤的深入研究提供科学依据。方法:采用常压耐缺氧、低浓度醋酸致小鼠腹腔毛细管通透性增高实验法,考察葛藤耐缺氧及抗炎作用;测定小鼠经口给药的最大耐受量。结果:葛藤小鼠最大耐受量为200g·kg-,为葛藤成人每日最大用量的400倍。葛藤提取液对小鼠1腹腔毛细血管通透性有明显抑制作用,并显示剂量依赖性,葛藤提取液与葛根提取液各组间无显著性差异。葛藤与葛根各剂量组均能延长小鼠常压耐缺氧存活时间,葛藤各剂量组与葛根对比,作用较强。结论:葛藤毒性较小,在抗炎与耐缺氧方面与葛根作用近似。

Objective:To explore the effects of Pueraiae lobatae caulis on anti-inflammation and anti-hypoxia activity compared with Pueraiae lobatae radix and assess the security of Pueraiae lobatae caulis for further study.Method:The hypoxia under normal pressure method and mice peritoneal inflammation induced by low concentrative acetic acid method were used to study its hypoxia and anti-inflammation activity.And the maximally tolerated dose of Pueraiae lobatae caulis for mice was determined.Result:The maximally tolerated dose of Pueraiae lobatae caulis for mice was 200g·kg-1,which was 400 times of andults' daily dose.Compared with normal saline group,the peritoneal inflammation swelling was inhibited remarkably by Pueraiae lobatae caulis in a dose-dependent manner.But no significant difference was observed when compared with the group of Pueraiae lobatae radix.Both Pueraiae lobatae caulis and Pueraiae lobatae radix could obviously prolong the living time of mice in hypoxia under the normal pressure,and Pueraiae lobatae caulis showed a stronger effect than Pueraiae lobatae radix.Conclusion:With low toxicity,Pueraiae lobatae caulis has similar effect on anti-hypoxia and anti-inflamma as Pueraiae lobatae radix.