450例宫颈活检组织中人乳头瘤病毒的分布特征

The Distribution and Infection of HPV in Cervical Biopsy Lesions among 450 Women

[目的]了解阴道镜检查患者的宫颈活检组织中HPV的感染率和亚型分布。[方法]对宫颈脱落细胞学异常的患者450例进行阴道镜下定位活检。活检组织同时行HPV基因分型和病理诊断,HPV基因分型采用斑点杂交方法进行。[结果]在450例宫颈活检患者中,其HPV感染率为65.3%。在HPV-DNA阳性患者中,检出率最高的HPV-16占36.7%,依次HPV-58为22.1%、HPV-18为18.7%、HPV-52为16.0%。在宫颈炎、CINⅠ、CINⅡ、CINⅢ中HPV-DNA阳性率分别为55.6%、69%、82.7%和88.9%。在宫颈炎、CINⅠ、CINⅡ,CINⅢ组织中均检测到HPV-16、-58、-18和-52。[结论]本研究中的宫颈活检组织中不仅检测到HPV-16和-18,还检测到HPV-58和-52感染,后者的发现为开发宫颈癌多价复合型疫苗提供了事实依据。

[Objective] To determine the distribution and infection of HPV in cervical cancer and precancerous lesions.[Methods] Four hundred patients with abnormal cytology were examined by colposcopy.HPV diagnosis was done by polymerase chain reaction using MY09/MY11 primers,for genotyping blot hybridization was used.The pathological results confirmed standards.[Result]A total of 294 women were HPV-DNA positive.HPV-16 was found in 36.7% of all women with positive HPV-DNA,HPV58 in 22.1%,HPV-18 in 18.7% and HPV-52 in 16.0%.The infection of HPV in lesions with cervicitis,CINⅠ,CINⅡ and CINⅢ were 55.6%,69%,82.7%and 88.9%,respectively.HPV-16,-58,-18 and-52 were found in different cervical lesions.[Conclusions] The finding of HPV-58 and-52 expects HPV-16 and-18 among Huzhou women with CINⅡand CINⅢ,and has important implications for the development of type-common HPV vaccines.